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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in several tumors, which leads to chromosome instability, and so may add to tumorigenesis. This protein was discovered to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important purpose in promoting mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two additional splice variants were being described but specific nucleotide sequence data just isn't however available.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged with the antibody.
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This guide outlines more doable optimization of mobile and immunoassay parameters to be certain the best possible results are...
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This tutorial outlines even further attainable optimization of mobile and immunoassay parameters to be certain the absolute best results are...
Antibodies sure to the blocking peptide not bind into the epitope to the target protein. By evaluating the staining in the blocked antibody vs . the antibody alone, one can see which staining is specific.
Goal Information and facts Cyclin E belongs for the really conserved cyclin loved ones, whose customers are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation designs which lead on the temporal coordination of each and every mitotic party. Cyclin E forms a posh with and capabilities as a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates for the G1-S period boundary which is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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